We use anti-RANK antibodies from R&D systems. Also, we have stained the
tartrate-resistant acis phosphatase enzyme using pararosaniline in acetate
buffer. the receipe is in the histonet archives. If you have difficulty
getting to it let me know and I'll email you a copy of the protocol.Good
luck, Donna Montague, M.S.
Research Associate
Physiology/Biophysics and Orthopaedic Surgery
University of Arkansas for Medical Sciences
(501) 603-1239


-----Original Message-----
From: Sarka Lhotak [mailto:lhotaks@mcmail.cis.mcmaster.ca]
Sent: Thursday, July 12, 2001 8:17 AM
To: histonet@pathology.swmed.edu
Subject: staining of mouse osteoclasts


Hello Histonetters,
	would anybody have a technique for staining osteoclasts in mouse
bones, demineralized and paraffin embedded? A source of an antibody
(calcitonin receptor, RANK ??) would be appreciated, or any other
suggestions.
	Thanks a lot,

Sarka Lhotak
Hamilton Regional Cancer Centre
McMa