We see that too, try blocking with avidin followed by biotin. Antigen
Retrieval somehow exposes biotin-like activity.
Abizar
www.innogenex.com

> -----Original Message-----
> From: Martha Ward [mailto:mward@wfubmc.edu]
> Sent: Friday, July 13, 2001 9:40 AM
> To: histonet@pathology.swmed.edu
> Subject: non-specific staining
>
>
> Martha Ward writes:
>
> We are experiencing non-specific staining on our negative control slides
> in some cases where we must use EDTA, pH 8 for our antigen retrieval
> solution.  We do not see this with every tissue and I haven't been able
> to pin down exactly why it is happening.  We use a Ventana ES for our
> staining and I was wondering if anyone has experienced this and what can
> I do to eliminate it.  Than