Paul

I have used 2 manufacturers c-fos antibodies on FFPE material. They are
1).the polyclonal from Oncogene (ab-2), Cat No. PC05 , and 2).a polyclonal
from santa cruz (K-25) Cat No. sc-253.

Both have worked extremely well on FFPE human CNS material as well as in
other species of CNS. In the case of the Oncogene we used it in sheep.,
Santa Cruz worked well with Guinea pig and mouse. Both needed antigen
retrieval with DAKO TRS solution.

I hope this helps

Jim Manavis
Senior Hospital Scientist
Department of Neuropathology
Institute of Medical & Veterinary Science
Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3668
FAX: 61-08-8222 3204
e.mail: jim.manavis@imvs.sa.gov.au
Disclaimer: Not this little black duck!

-----Original Message-----
From:	Paul Klosen [mailto:klosen@neurochem.u-strasbg.fr]
Sent:	Friday, 6 July 2001 6:28 pm
To:	histonet@pathology.swmed.edu
Subject:	c-fos antibodies

Hi,

We are currently trying to set up a series of experiments with c-fos
detection on formalin fixed paraffin embedded sections. The antibody we
were using so far on vibratome sections does not seem to work on FFPE
tissue. I would thus like to hear about your experience with different
c-fos antibodies that work on FFPE tissue (if there are any)

Thanx in advance

Paul
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Paul Klosen, PhD
CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
Universite Louis Pasteur  12, rue de l'Universite
F-67000 Strasbourg, FRANCE
Tel. 03.90.24.05.01  Fax. 03.90.24.05.28
========================klosen@neurochem.u-strasbg.fr=======================
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