Xylene is used in the coverslipping process therefore won't affect the
coverslip. I'm not sure about the Permount though. to remove plastic
coverslips without tissue stuck to them use acetone. soak the slides for
about 3 minutes, rinse in absolute alcohol, then xylene and re-coverslip.

Lynn Dize
Piedmont Hospital
Atlanta, GA

-----Original Message-----
From: Connelly.J. Keith [mailto:connelly@niehs.nih.gov]
Sent: Tuesday, July 03, 2001 2:07 PM
To: 'HISTONET@PATHOLOGY.SWMED.EDU'
Subject: Plastic Film Coverslip Repair


Fellow Histonetters,
Our Facility has a large number of H & E tissue slides that have
plastic "film" coverslips.  In a number of instances the tissues are
actually adhearing to the coverslip and are coming completely off the
slide.  One Histology Correspondent suggested it might not be
appropriate to use Xylene to repair these because it might liquify the
plastic film coverslip.  She also wasn't sure if Permount mounting
media would liquify the plastic film coverslip.  Any insight that
could be provided into successful resolution of this dilemma would be
great