RE: Plastic Film Coverslip Repair

From:Jill Songer <jtsonger@vt.edu>

I had the same trouble with the film coverslipper and NEVER used recycled 
xylene with it. I didn't have recycled xylene.




At 07:53 AM 7/5/2001 -0400, Weems, Joyce wrote:
>I've never used the plastic tape coverslippers, but seeing all this----I
>wonder if the sites having the problem used recycled xylene on the
>coverslipper........ When we had the demo our rep stressed the fact that we
>were only to use new xylene.
>
> > -----Original Message-----
> > From: Aidan Schurr [SMTP:Aidan.Schurr@hvh.co.nz]
> > Sent: Tuesday, July 03, 2001 9:03 PM
> > To:   connelly@niehs.nih.gov; histonet@pathology.swmed.edu
> > Subject:      Re: Plastic Film Coverslip Repair
> >
> > Hi,
> >
> > I've posted this several times before here.  The best method I've found
> > for repairing these is to cut around the tissue, through the coverslip,
> > with a scapel blade, then coverslip with regular mounting media and a
> > glass coverslip.  This will even work where the coverslip has broken the
> > tissue and left some on the slide and some on the 'slip.  Just carefully
> > position the cut coverslip in a little media in the area where the
> > remaining tissue is, then coverslip as above.  You can reposition slightly
> > under here by moving the glass coverslip.
> >
> > Whew!!!
> >
> > Good Luck
> > Aidan
> >
> >
> > __
> >
> > aidan schurr  b.m.l.sc
> > section head, histology
> > hutt valley district health board
> > lower hutt
> > new zealand
> >
> > aidan.schurr@hvh.co.nz
> > ++64 4 570 9173
> >
> > >>> "Connelly.J. Keith" <connelly@niehs.nih.gov> 6:07:09 a.m. Wednesday, 4
> > July 2001 >>>
> > Fellow Histonetters,
> > Our Facility has a large number of H & E tissue slides that have
> > plastic "film" coverslips.  In a number of instances the tissues are
> > actually adhearing to the coverslip and are coming completely off the
> > slide.  One Histology Correspondent suggested it might not be
> > appropriate to use Xylene to repair these because it might liquify the
> > plastic film coverslip.  She also wasn't sure if Permount mounting
> > media would liquify the plastic film coverslip.  Any insight that
> > could be provided into successful resolution of this dilemma would be
> > greatly appreciated.
> > Sincerely,
> > J. Keith Connelly
> >
> >


**************************************************************************
Jill Songer HT (ASCP)
Supervisor, Anatomic Pathology
Virginia Tech
Veterinary Medical Teaching Hospital






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