RE: Plastic Film Coverslip Repair

From:"Weems, Joyce" <JWEEMS@sjha.org>

I've never used the plastic tape coverslippers, but seeing all this----I
wonder if the sites having the problem used recycled xylene on the
coverslipper........ When we had the demo our rep stressed the fact that we
were only to use new xylene. 

> -----Original Message-----
> From:	Aidan Schurr [SMTP:Aidan.Schurr@hvh.co.nz]
> Sent:	Tuesday, July 03, 2001 9:03 PM
> To:	connelly@niehs.nih.gov; histonet@pathology.swmed.edu
> Subject:	Re: Plastic Film Coverslip Repair
> 
> Hi,
> 
> I've posted this several times before here.  The best method I've found
> for repairing these is to cut around the tissue, through the coverslip,
> with a scapel blade, then coverslip with regular mounting media and a
> glass coverslip.  This will even work where the coverslip has broken the
> tissue and left some on the slide and some on the 'slip.  Just carefully
> position the cut coverslip in a little media in the area where the
> remaining tissue is, then coverslip as above.  You can reposition slightly
> under here by moving the glass coverslip.
> 
> Whew!!!
> 
> Good Luck
> Aidan
> 
> 
> __
> 
> aidan schurr  b.m.l.sc
> section head, histology
> hutt valley district health board
> lower hutt
> new zealand
> 
> aidan.schurr@hvh.co.nz
> ++64 4 570 9173
> 
> >>> "Connelly.J. Keith" <connelly@niehs.nih.gov> 6:07:09 a.m. Wednesday, 4
> July 2001 >>>
> Fellow Histonetters,
> Our Facility has a large number of H & E tissue slides that have
> plastic "film" coverslips.  In a number of instances the tissues are
> actually adhearing to the coverslip and are coming completely off the
> slide.  One Histology Correspondent suggested it might not be
> appropriate to use Xylene to repair these because it might liquify the
> plastic film coverslip.  She also wasn't sure if Permount mounting
> media would liquify the plastic film coverslip.  Any insight that
> could be provided into successful resolution of this dilemma would be
> greatly appreciated.
> Sincerely,
> J. Keith Connelly
> 
> 




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