Vysis recommends 24 - 48 hours. How many of us can do that?!?!?!

> -----Original Message-----
> From:	Priscilla [SMTP:pdelvent@wyoming.com]
> Sent:	Wednesday, July 04, 2001 6:35 PM
> To:	HistoNet Server
> Subject:	Re: Daily Digest
> 
> While we are on the subject of fixation for FISH and Her/2Neu.  (Also ER
> and
> PR ) Are there any documentation/s of how long a tissue should be fixed
> for
> these studies?  I maintain that the  usual 2 hours of fixation on the
> processor is not adequate time to fix larger pieces of tissue, especially
> breast tissue.  Of course there is some fixation during the day before
> processing, however, I have always believed most fatty tissue needs at
> least
> overnight fixation for adequate processing.  Does the time element
> correlate
> with the receptor response?
> Thanks - Priscilla in Central Wyo.
>