RE: Cryostat sectioning

From:Cathy Gorrie <C.Gorrie@unsw.edu.au>

<!doctype html public "-//W3C//DTD W3 HTML//EN"> <html><head><style type="text/css"><!-- blockquote, dl, ul, ol, li { margin-top: 0 ; margin-bottom: 0 } --></style><title>RE: Cryostat sectioning</title></head><body> <div>Firstly, I would check that<u> everything</u> is done up tight. All the problems you're described can happen when something is loose.</div> <div>Cathy</div> <div><br></div> <div>At 11:26 AM -0400 4/7/01, FANDORFP@aol.com wrote:</div> <blockquote type="cite" cite><font face="Arial" size="-1">I have been having some touble while sectioning fresh frozen tissue this<br> week. I have tried several different things to rememdy, nothing working. Has<br> anyone else experienced these problems? And how where they resolved.<br> 1. The tissue is splitting in half, all the way across the face of the block.<br> Not the up and down which would indicate to move the blade.<br> 2. Bad problem with thick and thin sections. I've sprayed the tissue to bring<br> down the temp and make it colder.<br> 3. Also when facing the block I have noticed, that there are ripples in the<br> block.<br> 4. Alot of Chatter, and the venetian blind.<br> So anyone out there can help!!!!! I appreciate it!!! Thank -you ..</font></blockquote> <div><br></div> <div>-- <br> ---------------------------------------------------<br> Cathy Gorrie<br> Scientific Officer<br> Neural Injury Research Unit,<br> School of Anatomy,<br> University of New South Wales<br> Sydney, N.S.W. 2052<br> <br> Phone: 61 2 9385 2462<br> Fax  : 61 2 9313 6252<br> e-mail: c.gorrie@unsw.edu.au<br> </div> </body> </html>
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