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<div>Firstly, I would check that<u> everything</u> is done up tight.
All the problems you're described can happen when something is
loose.</div>
<div>Cathy</div>
<div><br></div>
<div>At 11:26 AM -0400 4/7/01, FANDORFP@aol.com wrote:</div>
<blockquote type="cite" cite><font face="Arial" size="-1">I have been
having some touble while sectioning fresh frozen tissue this<br>
week. I have tried several different things to rememdy, nothing
working. Has<br>
anyone else experienced these problems? And how where they
resolved.<br>
1. The tissue is splitting in half, all the way across the face of the
block.<br>
Not the up and down which would indicate to move the blade.<br>
2. Bad problem with thick and thin sections. I've sprayed the tissue
to bring<br>
down the temp and make it colder.<br>
3. Also when facing the block I have noticed, that there are ripples
in the<br>
block.<br>
4. Alot of Chatter, and the venetian blind.<br>
So anyone out there can help!!!!! I appreciate it!!! Thank -you
..</font></blockquote>
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<div>-- <br>
---------------------------------------------------<br>
Cathy Gorrie<br>
Scientific Officer<br>
Neural Injury Research Unit,<br>
School of Anatomy,<br>
University of New South Wales<br>
Sydney, N.S.W. 2052<br>
<br>
Phone: 61 2 9385 2462<br>
Fax : 61 2 9313 6252<br>
e-mail: c.gorrie@unsw.edu.au<br>
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