RE: Cryostat sectioning
--part1_114.12817f5.28748fb5_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit
I have been having some touble while sectioning fresh frozen tissue this
week. I have tried several different things to rememdy, nothing working. Has
anyone else experienced these problems? And how where they resolved.
1. The tissue is splitting in half, all the way across the face of the block.
Not the up and down which would indicate to move the blade.
2. Bad problem with thick and thin sections. I've sprayed the tissue to bring
down the temp and make it colder.
3. Also when facing the block I have noticed, that there are ripples in the
block.
4. Alot of Chatter, and the venetian blind.
So anyone out there can help!!!!! I appreciate it!!! Thank -you ..
--part1_114.12817f5.28748fb5_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit
<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>I have been having some touble while sectioning fresh frozen tissue this
<BR>week. I have tried several different things to rememdy, nothing working. Has
<BR>anyone else experienced these problems? And how where they resolved.
<BR>1. The tissue is splitting in half, all the way across the face of the block.
<BR>Not the up and down which would indicate to move the blade.
<BR>2. Bad problem with thick and thin sections. I've sprayed the tissue to bring
<BR>down the temp and make it colder.
<BR>3. Also when facing the block I have noticed, that there are ripples in the
<BR>block.
<BR>4. Alot of Chatter, and the venetian blind.
<BR>So anyone out there can help!!!!! I appreciate it!!! Thank -you ..</FONT></HTML>
--part1_114.12817f5.28748fb5_boundary--
<< Previous Message | Next Message >>