Angeline,
We are working with a BrDU model and the optimum time to harvest tissue is
24 hours post injection. This information came from the manufacturer of the
BrDU. 
Best of luck!
Mary Georger	
University of Rochester
Center for Cardiovascular Research

> ----------
> From: 	J. A. Kiernan
> Sent: 	Wednesday, July 4, 2001 1:23 AM
> To: 	Angeline Martin-Studdard
> Cc: 	Histonet
> Subject: 	Re: BrdU  (and how and who to ask)
> 
> Your email was addressed to me alone, and I cannot
> give you any useful suggestions.
> 
> For expert advice you should address your question to the
> HistoNet listserver. Your email indicates that you subscribe
> to that listserver. Probably someone looking at today's 
> Histonet questions will be able to help you. I do not know
> the answer to your question, and I do not intend to go to
> the library and occupy an hour looking it up. 
> 
> Histonet is a useful resource for everyone in the field, 
> especially those who do not have a library or a shelf of 
> textbooks. Anyone can answer a question. All can read
> the answer, and some will make comments that support,
> supplement, modify, contradict etc. Questions and replies
> are sent to everyone. Anyone can chip in with an answer
> or a suggestion. You will learn more from this than 
> from picking out a regular answerer (me, about whom you
> obviously know nothing) to ask a question that might
> interest many other Histonetters. 
>  
> I'm forwarding this reply to Histonet at large because
>  (a) it's of geenral interest, and
>  (b) someone reading it might effortlessly be able to 
>      give you an answer to your question.
> 
> John Kiernan
> London, Canada. 
>    kiernan@uwo.ca
>    http://publish.uwo.ca/~jkiernan
> -----------------------------
> On Tue, 3 Jul 2001, Angeline Martin-Studdard wrote:
> > Date: Tue, 03 Jul 2001 16:07:48 -0400
> > 
> > Dear Dr. Kiernan,
> > 
> > My name is Angeline Martin and I am employed at the Medical College of
> Georgia.  I have had the pleasure of reading and utilizing your advice
> provided on the histonet many times.  Our projects have involved the use
> of BrdU and I have been unable to obtain clarification on precisely how
> long this will be expressed in cells.  We inject BrdU (IP) into young mice
> who have undergone some level of ischemia.  The BrdU is easily detectable
> in the brain tissue sections.  However, my concern is whether we are
> observing all new cell development from injection up until the time of
> sacrifice or is there only a "window" of opportunity for each cell in
> which the BrdU will be detectable?
> > 
> > Any help you can provide will be greatly appreciated.
> > 
> > Sincerely,
> > 
> > Angeline M. Martin
> > Department of Neurology
> > Medical College of Georgia
> > 1459 Laney-Walker Blvd.
> > Augusta, GA 30912
> > Ph: 706-721-6969
> > Fax: 706-721-6839
> > Amartin@mail.mcg.edu
> > 
> ----------------------------------------
> 
> 
>