PPAR gamma

From:Sylvia Poulos <spoulos@saa.ars.usda.gov>

Has anyone done staining with PPARgamma antibodies and found discrepancies in the results? I've used both polyclonal and monoclonal (From Affinity and Santa Cruz) and get great staining with them.  The kicker is this... when we do western blots with the same antibodies the results are very different that the staining.  We'll see high amounts in staining but not on the westerns with all four antibodies.  This happened in tissue and in cultured cells.

Any thoughts as to why this may be happening?
Drowning in thought... S

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