Hi folks,
Several enzyme histochemical methods e.g. for dehydrogenases use NBT as the
chromogen.At the end of the development of the formazan reaction
product,several methods recommend fixing for 10-15 mins in 10% formaldehyde
then dehydating through alcohols,clearing in xylene and mounting.I find
this loses some of the reacion product and the results are weaker than if I
aqueous mount.Does anyone routinely dehydrate (especially after using
NBT/BCIP as a chromogen and substrate combination)?How can I preserve the
colour better?Dehydrating and mounting make photography so much better.

Allan (on a hijacked compu