[Fwd: stereology on thick sections]

From:Karen Pawlowski <kpawlow@swbell.net>

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I thought this might be a better forum to help this person out. And I'd
love to know how people section blocks of glycol methacrylate at 
20 microns.  

Karen Pawlowski, Ph.D.
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Date: Mon, 09 Jul 2001 23:18:34 -0400 (EDT)
From: "CachatF@aol.com"@sparc5.microscopy.com
Subject: stereology on thick sections
To: Microscopy@sparc5.microscopy.com
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I am interested in counting the total number of glomeruli in the kidneys in 
> rats with obstructive uropathy. To perform stereology, I have to cut thick 
> sections at 20 micrometers. I found that the kidney itself breaks down and 
> get very damaged at this thickness (kidneys fixed in 10% formalin and 
> embedded in parrafin), probably because the kidney is much harder than the 
> paraffin. In the litterature, some people embed kidneys in glycol 
> methacrylate. It might be better (harder), but then I do not know how to 
> my sections which are about 2.5 to 3 cm long, if I need a glass/diamond 
> that long. Any ideas about cutting thick sections in paraffin?, or is it 
> worth re-embedding in glycolmethacrylate, and any ideas about cutting my 
> serial sections at 20 micrometers in that resin?
> Thank you a lot for your help!
> F. Cachat
> University of Virginia
> fc6b@virginia.edu


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