Fluorescence & background
|From:||Sylvia Poulos <email@example.com>|
I'm about to do some immuno-staining and want to use FITC and TRITC labelled antibodies. I've heard that fixing cells with 4% paraformaldehyde may give me pretty high background and may interfere with the staining. Does anyone know if that's true, or why that happens? Is there a different fixative that I can use that would prevent this?
Thanks for the help!
USDA-ARS-Animal Physiology Rese
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