Cytokine Immunohistochemistry

From:Liz Sabin <>

Hello all,
Does anyone have any experience using tyramide amplification 
immunohistochemical techniques to detect cytokine expression in 
paraffin sections of, for example, human skin?   Did you have any 
problems optimizing the method -  background speckling for 

Any responses gratefully received.
Liz Sabin
Edinburgh Royal Infirmary.

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