Brazilin
From: | Paul Klosen <klosen@neurochem.u-strasbg.fr> |
Hi,
A few weeks ago, I read about using brazilin instead of hematoxylin for a
red nuclear stain and somebody (I apologize, I don't remember anymore who)
suggested to prepare the stain like hematoxylin by replacing henatoxylin
with Brazilin. I did this with our protocol for Mayers hemalun (1 g
hematoxylin in 250 ml ssium alum, then adding 8 ml glacial acetic acid and
150 ml glycerol and water to 500 ml, oxidized with 0.1 g sodium iodide).
Unfortunately the stain does not work.
Can anyone post a full protocol for this Brazilin stain.
Thanx in advance
Paul
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Paul Klosen, PhD
CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
Universite Louis Pasteur 12, rue de l'Universite
F-67000 Strasbourg, FRANCE
Tel. 03.90.24.05.01 Fax. 03.90.24.05.28
========================klosen@neurochem.u-strasbg.fr=========================
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