whole mouse imaging in real time

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
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A group at Stanford uses a CCD camera and a luciferase-activating substrate
to perform 
"Real time in vivo imaging of T cell trafficking in autoimmune disease"
poster #149.23 at the Seattle 2000 FASEB meeting.  Sandora MR, Costa GL,
Benson JM, Lejon K, Slavin AJ, Contag CH and Fathman CG. 

Hopefully they have publications which can be located via PUBMED describing
this method in detail.  

This permits a systemic delivery of the substrate, and whole animal (live!)
was imaged, which visualized all organs at the same time, defining the
kinetics of T cell trafficking.  

The camera setup probably is considerably cheaper and smaller than a huge
cryostat, Instrumedics Cryojane Tape Transfer setup, plus all the work of
just cutting sections through a whole mouse.  PLUS a mouse could be tested
(in vivo) at different time intervals, time and cost saving, versus
adoptive transfer methods that require euthanasia of mice at time intervals
(ex vivo). 

Very elegant!

After pricing cryostats, a monster Leica or Polycut, lack of space for
housing the instrument and all the pricey extras and just the time involved
in serial or semi serial sectioning a mouse!!! we are looking into the CCD
setup instead.  
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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