tissue processing problem

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From:"L. Gibbs" <lgibbs@u.washington.edu>
To:Histonet <HistoNet@pathology.swmed.edu>
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Can you fix the skin overnight? (Although 4 hours is probably
adequate.) I usually let my rodent tissue sit in 70% ETOH for at
least a couple hours before going through a processing schedule
of 30-45 minutes per solution...I would go with 45 minutes if the
skin contained a lot of adipose. I must also confess to never
washing the after the fixation step.
Good luck!

P-Bio Histology

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