Dear List:

     A colleague needs advice on sectioning fruit fly legs for light
microscopy. Frozen sections perfered but paraffin is acceptable. There
is a lot of chitin and little soft tissue. Is a knife with a special
edge called for? Harder than normal wax? Colder than normal cutting temp
for frozens? All suggestions are welcomed!

TIA,
Geoff
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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