peripheral nerve staining

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From:Louise Taylor <>
To:histonet <>
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Hi Histonetters,

Seeing as there have been some queries regarding  neuropathology I thought I
might as well jump on  the bandwagon myself with a question.
A colleague is trying to demonstrate peripheral nerves in 2 day old rats for
subsequent image analysis. I have tried S100, but have got variable results
as well as some tissue damage due to microwave antigen retrieval (poor
quality IHC without HIER) so.... can anyone suggest something that might be
more useful? Perhaps a special stain? One of the old fashioned silver ones?

looking forward to your suggestions
Louise taylor

Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
South Africa

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