Re microwave processing
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From: | a i d a n s c h u r r <Aidan.Schurr@hvh.co.nz> |
To: | Clarke Ian <clarke.ian@virgin.net>, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Hi Ian,
The microwave energy should dramatically speed up the fixation
process for formalin. In aqueous solution formaldehyde exists
mostly as methylene glycol, in equilibrium with monomeric
formaldehyde. The microwave energy actually forces the
equilibrium back to the left, creating dramatically more free
formaldehyde (the bit that actually does the fixation). The net
effect is that you should be able to fix a bit of tissue as you
describe in about five minutes... the trick is to be patient enough to
allow the formalin to penetrate the tissue - this you cannot speed
up with the microwave!
Hope this is some help.
Aidan
> Could anyone tell us what the microwave processor is
> actually contributing to the finished block.We think that all we are using
> the microwaves for is as a means to quickly heat up the dehydrating
> solutions and wax as we have not seen any effect on fixation .We are in the
> process of just heating the solutions and adding the block to them for the
> same time they where in the processor to see if this would give us the same
> results.
> I would appreciate any thoughts people may have on these
> findings and any recent papers on the subject.
>
> Ian Clarke
> Histopathology/Cytopathology department
> Craigavon Area Hospital
> 66 Lurgan Road
> BT66 5QQ
> Northern Ireland.
>
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___________________________________________________
shin: device for finding furniture in the dark...
___________________________________________________
a i d a n c s c h u r r
mlso, histology department
hutt valley health
lower hutt, new zealand
ph. ++64 4 5709173
fax ++64 4 5709214
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