Transgenic T cells were transduced with a retrovirus, to express the
luciferase, and these cells were then adoptively transferred into
autoimmune susceptible mice.  
Luciferase -activating substrate was delivered systemically, and the animal
imaged. 
It was nice for both visualizing all organs and study the kinetics of cell
trafficking to autoimmune inflammations sites.  

Sorry, I have no info on the imaging set up to date.



At 12:51 PM 7/24/00 -0700, you wrote:
>hi gayle, thanks for the reference! Though I think the approach would only
work
>with transgenic mice that have an introduced luciferase gene. Do you have
more
>details about the imaging set up?
>abizar
>www.innogenex.com
>1-877-igx-info
>
>Gayle Callis wrote:
>
>> A group at Stanford uses a CCD camera and a luciferase-activating substrate
>> to perform
>> "Real time in vivo imaging of T cell trafficking in autoimmune disease"
>> poster #149.23 at the Seattle 2000 FASEB meeting.  Sandora MR, Costa GL,
>> Benson JM, Lejon K, Slavin AJ, Contag CH and Fathman CG.
>>
>> Hopefully they have publications which can be located via PUBMED describing
>> this method in detail.
>>
>> This permits a systemic delivery of the substrate, and whole animal (live!)
>> was imaged, which visualized all organs at the same time, defining the
>> kinetics of T cell trafficking.
>>
>> The camera setup probably is considerably cheaper and smaller than a huge
>> cryostat, Instrumedics Cryojane Tape Transfer setup, plus all the work of
>> just cutting sections through a whole mouse.  PLUS a mouse could be tested
>> (in vivo) at different time intervals, time and cost saving, versus
>> adoptive transfer methods that require euthanasia of mice at time intervals
>> (ex vivo).
>>
>> Very elegant!
>>
>> After pricing cryostats, a monster Leica or Polycut, lack of space for
>> housing the instrument and all the pricey extras and just the time involved
>> in serial or semi serial sectioning a mouse!!! we are looking into the CCD
>> setup instead.
>> Gayle Callis
>> Veterinary Molecular Biology
>> Montana State University
>> Bozeman MT 59717-3610
>> 406 994-4705
>> 406 994-4303
>
>
>
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303