Companies like Vector have one year expiration dates on their avidin biotin
reagents, but I am not sure IF they put some type of preservative in the
reagent.  You could ask them and/or check their MSDS/specification sheets,
and try to duplicate it.    

  

At 06:35 AM 7/31/00 -0700, you wrote:
>Thanks to all who responded weeks ago to my question
>about avidin/biotin blocking reagents from
>concentrates..the information was very helpful.  I
>have couple more questions for everyone.
>
>1- What is the average shelf life for the reagents
>when made from concetrate if stored properly?
>
>2- Is anyone using these reagents in a large
>volume(i.e. staining dish) and can the solution be
>used for more than one rack of slides?  We currently
>apply to each slide during ihc protocol.
>
>Amy S. Porter, HT(ASCP)
>Michigan State University
>College of Human Medicine
>
>
>
>=====
>Amy S.Porter, HT(ASCP)
>Michigan State University
>College of Human Medicine
>portera203@yahoo.com
>
>__________________________________________________
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>
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303