Re: quenching autofluorescence
<< Previous Message | Next Message >>
From: | Margaret Gondo <mgondo@popmail.opt.uh.edu> |
To: | Mike King <making@nersp.nerdc.ufl.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Do you know if the 0.5% Sodium borohydride solution should be adjusted
to a neutral pH ~7?
Thanks,
Margaret
Mike King wrote:
>
> Yesterday,"Barry, Lilith" <Lilith.Barry@nrc.ca> posted
> Subject: Quenching autofluorescence
> ...Is there a way of quenching or blocking this autofluorescence before doing
> the immuno? ...
>
> In the Molecular Probes catalog there is a procedure for this, I don't have
> the catalog at the moment but I believe it is simple immersion in 0.5%
> sodium borohydride for a few minutes prior to antibody incubations. You
> could test it and work out the necessary reaction times on sections not
> being immunolabelled.
<< Previous Message | Next Message >>