Re: peripheral nerve staining

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From:Geoff McAuliffe <mcauliff@UMDNJ.EDU>
To:Louise Taylor <>
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Louise Taylor wrote:

> Hi Histonetters,
> Seeing as there have been some queries regarding  neuropathology I thought I
> might as well jump on  the bandwagon myself with a question.
> A colleague is trying to demonstrate peripheral nerves in 2 day old rats for
> subsequent image analysis. I have tried S100, but have got variable results
> as well as some tissue damage due to microwave antigen retrieval (poor
> quality IHC without HIER) so.... can anyone suggest something that might be
> more useful? Perhaps a special stain? One of the old fashioned silver ones?
> looking forward to your suggestions
> Louise taylor
> Research Laboratory
> Department of Anatomical Pathology
> South African Institute for Medical Research
> Johannesburg
> South Africa

How about good old H&E? I teach histology and peripheral nerves look fine in H&E
in our (commercially made) wax sections.

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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