Re: fast red
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From: | Barry Rittman <brittman@mail.db.uth.tmc.edu> |
To: | histology <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Betsy,
No.
Nuclear fast red is the better stain to use as it give a cleaner nuclear
stain with less background but requires an aluminum mordant
Dissolve 0.1 gms nuclear fast red in
a 5% aqueous solution of aluminum sulfate by boiling.
Cool.
Filter.
The stain lasts for several weeks.
Stain sections from distilled water for 5-10 mins or longer (difficult to
overstain)
The stain is alcohol fast
Barry
"Molinari, Betsy" wrote:
> Is neutral red the same as nuclear fast red? I am doing a Perls Iron
> stain and have two protocols one says nuetral red and the other nuclear
> fast red as counterstain.
> Thanks.
> Betsy Molinari
> Texas Heart Institute
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