Re: digital photomicrography

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From:Kappeler Andreas <>
To:Histonet <>
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Dear Yuri

We have recently had the new Zeiss AxioCam for a demonstration. Its
performance was superb, better than any of the digitals we have at our
institute. Main difference: the digitals we currently use are modified
consumer cameras (Olympus DP10, Nikon Coolpix), have 1.3 MPx, and didn't
cost a fortune. Their suitability for immunofluorescence is limited, but for
everyday documentation of slides viewed with conventional light microscopy
they are okay; many of our researchers produce their (light microscopy) pics
for publications with these cameras. However, if you are looking for a
professional camera designed for use with a microscope, shop for the Zeiss
AxioCam, but don't forget the BIG purse ... The AxioCam has a Peltier-cooled
chip and is very well suited for both, immunofluorescence and light
microscopy. It is very sensitive, which results in reasonably short exposure
times for fluorescence (limited bleaching problems). It allows on-line
viewing on your computer screen, is in the 3 Mpx range, has automatic white
compensation and many other features that make working with it very
pleasant. Only drawback: my budget is limited ... (I hope yours is not!).

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

Original message:
> Dear Histonetters,
> Does anyone recommend good 3 (or less? or more?) megapix digital
> photomicrography unit for light and immunofluorescence microscopy?
> With thanks in advance
> Dr Yuri A Krivolapov MD
> Associate Professor in Histopathology
> St.-Petersburg Medical Academy of Postgraduate Studies and
> Head of Laboratory of Immunohistochemistry
> Leningrad Region Bureau of Pathology
> St.-Petersburg Russia

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