Re: anti-rat TNF-alpha and anti-rat IL-6
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| From: | Marcia Bentz <mb7x@virginia.edu> |
| To: | Cathie Crukley <Cathie.Crukley@sjhc.london.on.ca>, histonet@pathology.swmed.edu |
| Reply-To: | |
| Content-Type: | text/plain; charset=us-ascii |
Don't know if Pharmingen has anti-rat for these cytokines, but I use their
rat anti-mouse TNF and IL6 on paraffin sections with HIER (Vector antigen
retrieval solution). The results are not spectacular in mouse kidney, but
spleen as positive control looks great.
Marcia
At 12:22 PM 07/25/2000 -0400, Cathie Crukley wrote:
>
>Does anyone know if these antibodies can be used on paraffin? I have
tried the antibodies from R&D Systems using HIER, enzyme digestion and
untreated without success. Is there another supplier someone is using?
>
>Thanks,
>
>Cathie Crukley
>SJHC London, Ontario
>
>
>
>
Marcia Bentz
Lab Specialist Sr
University of Virginia
Medicine/Nephrology
mb7x@virginia.edu
804-982-1069
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