Re: anti-rat TNF-alpha and anti-rat IL-6

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From:Marcia Bentz <>
To:Cathie Crukley <>,
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Don't know if Pharmingen has anti-rat for these cytokines, but I use their
rat anti-mouse TNF and IL6 on paraffin sections with HIER (Vector antigen
retrieval solution). The results are not spectacular in mouse kidney, but
spleen as positive control looks great.


At 12:22 PM 07/25/2000 -0400, Cathie Crukley wrote:
>Does anyone know if these antibodies can be used on paraffin?  I have
tried the antibodies from R&D Systems using HIER, enzyme  digestion and
untreated without success.  Is there another supplier someone is using?
>Cathie Crukley
>SJHC London, Ontario
Marcia Bentz
Lab Specialist Sr
University of Virginia

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