Re: Two Questions

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From:Abizar Lakdawalla <>
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We have an ISH kit that is pretty easy to use. It contains ready-to-use reagents.
The manuals are available on the web site or can fax you a copy.
1-877-igx-info wrote:

> I have two questions for all of you.  Does anyone have any suggestions for an
> antibody for beta-galactosidase in rat brain sections in paraffin?  I have
> b-gal transfected human tumor cell spheroids implanted in rat brains and want
> to demonstrate those cells however much of a mass they may have formed.
> there a step by step, idiot proof ISH (DNA) protocol, complete with
> formulae for all solutions required, in existence somewhere?  This is the
> nonthinking approach to doing something when you have no time to work on it
> in detail.  All suggestions will be appreciated.
> Thanks,
> Nancy Lemke
> Henry Ford Hospital

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