Re: Two Questions

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From:Luis Chiriboga <>
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Try mouse (clone 2e9, cat # K86409M) or rabbit (cat # B59136R) anti-beta
Galactosidase (E.coli) from  Biodesign International (,
207-985-1944).  I strongly recommend a good positive control (preferably your
transfected tumor cells).  If you do not get staining in your brain samples,  try
using an amplification system (Dako CSA or NEN tyramide).

For a protocol, Pay a visit to Protocol Online at:

Hope this helps wrote:

> I have two questions for all of you.  Does anyone have any suggestions for an
> antibody for beta-galactosidase in rat brain sections in paraffin?  I have
> b-gal transfected human tumor cell spheroids implanted in rat brains and want
> to demonstrate those cells however much of a mass they may have formed.
> there a step by step, idiot proof ISH (DNA) protocol, complete with
> formulae for all solutions required, in existence somewhere?  This is the
> nonthinking approach to doing something when you have no time to work on it
> in detail.  All suggestions will be appreciated.
> Thanks,
> Nancy Lemke
> Henry Ford Hospital

Luis Chiriboga Ph.D HT(ASCP)QIHC
Department of Pathology 4W27
Bellevue Hospital
NYU School of Medicine
First Avenue & 27th Street
New York, NY 10016

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