Re: Two Questions
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From: | Luis Chiriboga <luis.chiriboga@med.nyu.edu> |
To: | Nperson211@aol.com |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Try mouse (clone 2e9, cat # K86409M) or rabbit (cat # B59136R) anti-beta
Galactosidase (E.coli) from Biodesign International (www.biodesign.com,
207-985-1944). I strongly recommend a good positive control (preferably your
transfected tumor cells). If you do not get staining in your brain samples, try
using an amplification system (Dako CSA or NEN tyramide).
For a protocol, Pay a visit to Protocol Online at:
http://www.protocol-online.net/
Hope this helps
Nperson211@aol.com wrote:
> I have two questions for all of you. Does anyone have any suggestions for an
> antibody for beta-galactosidase in rat brain sections in paraffin? I have
> b-gal transfected human tumor cell spheroids implanted in rat brains and want
> to demonstrate those cells however much of a mass they may have formed.
> Also...is there a step by step, idiot proof ISH (DNA) protocol, complete with
> formulae for all solutions required, in existence somewhere? This is the
> nonthinking approach to doing something when you have no time to work on it
> in detail. All suggestions will be appreciated.
> Thanks,
> Nancy Lemke
> Henry Ford Hospital
--
Luis Chiriboga Ph.D HT(ASCP)QIHC
Department of Pathology 4W27
Bellevue Hospital
NYU School of Medicine
First Avenue & 27th Street
New York, NY 10016
(212)562-4667
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