Re: Revisiting storage of cryosections

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From:Geoff McAuliffe <mcauliff@UMDNJ.EDU>
To:Greg Dobbin <dobbin@Upei.CA>
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Greg Dobbin wrote:

> Hello Histonetters,
> I do recall this subject being discussed before, but at the time I
> read with passing interest. I now have a graduate student
> wondering how long an air-dried frozen section can be stored and
> under what conditions, prior to doing immunoelectron microscopy.
> He would like to batch process his sections. Any suggestions?
> Cheers! Greg
> >
> >Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 Unviversity Ave.
> Charlottetown, P.E.I.
> Canada,  C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851

    I would not air dry. I have done immunostaining (light microscope
level) for GFAP, tyrosine hydroxylase, dopamine beta-hyroxylase and
various neuropeptides sections stored for weeks in PBS in the
refrigerator. Air dried sections lost immunoreactivity in a few days.
    Your mileage may vary.

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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