Our Pathologists prefer the Rapid Pap stain.  Well worth the trouble and the
extra minute to stain.  We stain in Hematoxylin for 45 seconds and then dip
10 times in the rest of the solutions.

Dawn Schneider, HT(ASCP)
-----Original Message-----
From: Beth Cox <bethcox@tir.com>
To: Holland, Wayne <hollandwt@msx.upmc.edu>; histonet@pathology.swmed.edu
<histonet@pathology.swmed.edu>
Date: Friday, July 28, 2000 9:16 AM
Subject: Re: Rapid PAP stains for CT guided FNA Bx's


>To determine adequacy on FNA's,  most labs use either a Toluidine Blue wet
>film (the easiest method) or a Diff-Quik type stain.  Rarely do labs use a
>quick Pap stain (too much trouble).
>
>For the Toluidine Blue: Put a drop of the aspirate from the needle onto the
>slide, add a drop of Toluidine Blue on top of that, drop on a coverslip and
>look under the scope.  A preliminary determination of malignancy can often
>be made from this too.  Some labs just toss this slide afterwards, some
>gently slide off the coverslip, drop the slide in alcohol, and then stain
it
>with the rest of the FNA slides.
>
>A slide for Diff-Quik has to air dry before staining and sometimes this can
>take a minute or two, this can be frustrating.
>
>Obviously, I recommend doing a Toluidine Blue wet film.
>
>Beth Cox  SCT/HT(ASCP)
>
>
>----- Original Message -----
>From: "Holland, Wayne" <hollandwt@msx.upmc.edu>
>To: <histonet@pathology.swmed.edu>
>Sent: Thursday, July 27, 2000 10:56 AM
>Subject: Rapid PAP stains for CT guided FNA Bx's
>
>
>> Hello all,
>>
>> Can any one give info on, or procedure, or staining product for rapid
>> resulting
>> for CT FNA Bx's, to determine the adequacy of the specimen.
>>
>> Thanks in advance.
>>
>> Wayne T. Holland H.T (ASCP)
>> UPMC Braddock
>> 400 Holland Ave
>> Braddock, PA 15104
>> 412-636-5301 X6080
>>
>>
>>
>
>
>