Re: Quenching autofluorescence

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From:Shelley Sheridan <>
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<BR> <P>Are the particles that autofluoresce within the neurons?  Do you think it might be lipofucin?  Lipofucin is an aging pigment that is found predominately in the cytoplasm of cells and almost always will autofluoresce.  Just recently we obtained a paper in the Journal of Histochemistry and Cytochemistry Vol 47(6):  719-730, 1999 "Reduction of lipofuscin-like Autofluorsecence in Fluorescently Labeled Tissue".  In the paper they describe two treatment options used after staining that is supposed to reduce the autoflurosencence.  So we decided to try each option  Cupric Sulfate solution didn't work as well as the Sudan Black solution.  With 10% Sudan Black treatment the autofluorescence was significantly reduced at lower magnifications, but at higher magnifications didn't work as well.  We didn't have any problems with reduction of immunostaining and we only tested the procedure on human AD tissue.  I'm not sure how it will work in rodent.  I wo <P> </P><BR><BR><P>Shelley K. Sheridan<BR>Research Specialist<BR>Center for Neurodegenerative Disease Research<BR>University of Pennsylvania Medical School<BR>Philadelphia, PA 19104<BR>Phone:(215)614-0051 Fax: (215) 349-5909</P><p><br><hr size=1><b>Do You Yahoo!?</b><br> Get <a href="">Yahoo! Mail</a> - Free email you can access from anywhere!
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