We have used Prefer for about 6 years now.  When we started using it, there
were some changes we had to go through with the help of our pathologists.
It takes patients and persistence to develop your IHC protocols.  There are
no antibodies that we can't get to work... we have a 100% success rate!  We
also use almost all BioGenex antibodies and reagents with the OptiMax
automated stainer.

Mary Bryhan HT (ASCP)
NMH - Petoskey,  MI


----- Original Message -----
From: Amos Brooks <atbrooks@snet.net>
To: sharon lang <pathology3@hotmail.com>
Cc: <histonet@pathology.swmed.edu>
Sent: Saturday, July 22, 2000 9:50 PM
Subject: Re: Prefer fixative


> Hi,
>     I had wars with tissue fixed in this stuff. We had tissue sent from
many
> labs to us, often the tissue was fixed in Prefer sometimes they didn't
mention
> this. Firstly just give up on KI67 and Herceptest. Some tests stained
fine,
> others didn't unless you skipped antigen retrieval, while still others
required
> extra retrieval. It was a pain. Take it test by test if you must use it.
Stick
> to formalin if you can.
> by the way the H&Es look beautiful!
> Amos Brooks
>
> sharon lang wrote:
>
> > Dear Fellow Histonetters:
> > I am interested in any input from my colleagues regarding Prefer
Fixative.
> > If you use it, do you have to antigen retrieve..are your special stains
pale
> > and muddy???   Please help.
> > Sharon
> > The Cutting Edge
> > ________________________________________________________________________
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>
>
>