Re: Prefer fixative

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From:Amos Brooks <>
To:sharon lang <>
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    I had wars with tissue fixed in this stuff. We had tissue sent from many
labs to us, often the tissue was fixed in Prefer sometimes they didn't mention
this. Firstly just give up on KI67 and Herceptest. Some tests stained fine,
others didn't unless you skipped antigen retrieval, while still others required
extra retrieval. It was a pain. Take it test by test if you must use it. Stick
to formalin if you can.
by the way the H&Es look beautiful!
Amos Brooks

sharon lang wrote:

> Dear Fellow Histonetters:
> I am interested in any input from my colleagues regarding Prefer Fixative.
> If you use it, do you have to antigen retrieve..are your special stains pale
> and muddy???   Please help.
> Sharon
> The Cutting Edge
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