Re: Need supplier of Schiff's stain for Feulgen reaction

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From:Rena Fail <>
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Carol,  Are you using pararosanilin to make your Schiff's?

At 02:11 PM 7/17/00 -0400, you wrote:
>Hello Histonetters:
>I am tearing my hair out.  I have attempted to make Schiff's that will work
>in the Feulgen reaction (a routine stain in our lab) using 4 different lots
>of dry stain and 3 different recipes (Lilllie's cold, Coleman's hot, and
>Bancroft & Stevens), all to no avail.  Previously wonderful ready-made stain
>from our usual supplier no longer works (have tried 4 or 5 different lots).
>This supplier has recently changed lots of dry stain as well.  I have tried
>a second ready made stain to no avail.  All work fine in the PAS stain, but
>not Feulgen.
>Is there a vendor who can supply me with a Schiff's tested for Feulgen?
>Comments from John Kiernan, Samurai, and other Histonet gurus are most
>Regards -
>Carol B. McCollough, HT(ASCP)
>Diagnostics & Histology Laboratory Manager
>Maryland Department of Natural Resources
>Fisheries Service
>Cooperative Oxford Laboratory
>904 S. Morris Street
>Oxford, MD 21654

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