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Dear Melanie:

Zymed recommends trypsin digestion of FFPE tissue when using our kappa (clone:
HP6053) and lambda (clone: HP6054) antibodies.

Sincerely,

John Bliss
__


"Trivett Melanie (PMC)" wrote:

> Hello all,
>
> Was wondering if any kind souls out there could give me some advice on Kappa
> and Lambda staining on formalin fixed paraffin embedded human tissue.
> Currently we use tonsil as a control and use HIER by pressure cooking for 2
> minutes in citrate buffer (pH 6.0).  At the moment we get some background
> staining.  I have heard that some people use enzyme digestion for these
> antibodies...any comments??
>
> Thanks in advance.
>
> Melanie Trivett
> Peter MacCallum Cancer Institute
> Melbourne
> Australia

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