Do you use monoclonal or polyclonal antibody, and at what dilution?

-Teri
----- Original Message -----
From: <DDittus787@aol.com>
To: <TrivettMelanie@petermac.unimelb.edu.au>; <histonet@pathology.swmed.edu>
Sent: Wednesday, July 26, 2000 11:32 AM
Subject: Re: Kappa and Lambda IHC


> we use zymed antibodies with a protease digestion of 4 min, 6 min primary
> incubation and a pre-treatment of 3% h2o2 for 10 min , and get little to
no
> background.                                          Dana
>