Melanie,
  For years we had problems with our Kappa and Lambda IHC stains, but I 
think we have finally got it right!  We are using Dako LSAB2 detection kit 
and prediluted Dako Kappa & Lambda antibodies.  We do not perform HEIR as we 
do an enzyme pretreatment only (with Dako Proteinase K).  This gives us 
great, consistent results.  Good Luck!

Carla Shoffeitt
Columbus Regional Medical Center
Columbus, GA.


>From: "Trivett Melanie (PMC)" <TrivettMelanie@petermac.unimelb.edu.au>
>To: 'HistoNet' <histonet@pathology.swmed.edu>
>Subject: Kappa and Lambda IHC
>Date: Wed, 26 Jul 2000 16:40:56 +1000
>
>Hello all,
>
>Was wondering if any kind souls out there could give me some advice on 
>Kappa
>and Lambda staining on formalin fixed paraffin embedded human tissue.
>Currently we use tonsil as a control and use HIER by pressure cooking for 2
>minutes in citrate buffer (pH 6.0).  At the moment we get some background
>staining.  I have heard that some people use enzyme digestion for these
>antibodies...any comments??
>
>Thanks in advance.
>
>Melanie Trivett
>Peter MacCallum Cancer Institute
>Melbourne
>Australia
>

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