Re: IL-2 and frozen muscle

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From:Gayle Callis <>
To:Sue Danielson <>,
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Go into the R and D Systems website,, and pick up their
protocols for staining cytokines on frozen sections.  If you need technical
assistance, ask for Jennifer Haas, she can provide more answers on the
antibody.  Some people try cytokine staining using monoclonal antibodies
(BD Pharmingen has these) and R&D Systems has affinity purified polyclonals
which may also work.  R&D gives excellent info on the secondary antibodies
you may need, plus the dilution for these.    

The sections you are working with may work best with a formalin or
paraformaldehyde fixation, followed by a saponin buffer system.  R&D had a
Cytokine Detection by IHC booklet that gives references, etc, but the
website also provides the protocols.  

Good luck

At 09:54 AM 7/25/00 -0500, you wrote:
>Have just been asked to work on a project involving IHC for interleukin 2 on
>frozen skeletal muscle sections from human muscle biopsies.  So far a
>literature search has not proven very helpful.  If anyone out there has a
>suggestion or has an established method, I'd be interested!
>Thanks in advance,
>Susan Danielson, MS
>Neuromuscular Lab
>Dept. Neurology, Medical College of Wisconsin
>ph: 414.259.3836
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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