Re: IHC to see degenerative axon fibers
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Maria Mejia <maria@mail.ski.org> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Mon, 17 Jul 2000, Maria Mejia wrote:
> .... degenerating
> axon fibers, especially unmyelinated, in the mammalian brain (the
> primary visual cortex). I was thinking maybe a synaptophsin antibody,
> or perhaps a particular microtubules or neurofilament(s) antibody
> -which would be the best for the above?????
These are proteins that occur in _normal_ cells: synaptophysin
at synapses, neurofilament proteins in axons (except very thin
ones) and microtublules in all cells that have to move particles
within their cytoplasm. You may see abnormalities in degenerating
axons, but none of these will provide you with a selective
stain that picks out the degenerating axons. Older methods
(silver; especially Nauta-Gygax) can do this, but time after
axotomy is quite critical, as are all the steps in the preparation
and staining of the material.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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