Re: ER and Golgi
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Kenneth Chapman <kchapman@mail.med.upenn.edu> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Fri, 28 Jul 2000, Kenneth Chapman wrote:
> I am presently staining ER and Golgi on paraffin sections using
> Molecular Probes Concanavalin A and Wheat Germ Agglutinin Conjugates
> respectively. Has anyone ever done anything like this before?
Yes. In the early 1970s I had classical Golgi apparatus staining
with concanavalin A in neurons: a perinuclear distribution quite
different from the rough ER (Nissl) staining of the same cells.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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