Re: ER

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From:Cathie Crukley <>,
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Hi Carolyn, 

We use the ER antibody from Immunotech (Beckman/Coulter) clone 1D5. We antigen retrieve in citrate buffer and incubate overnight at 4 degrees, then use LSAB2 from Dako.  It works well at 1:100, with some cases we get some cytoplasmic staining, but as far as I know thats been documented.


SJHC, London, ON

>>> <> 07/19/00 02:55PM >>>

We are having problems with very weak staining with ER. We use antigen retrieval
in citrate buffer in the microwave.The antibody was from Nova-Castra and then we
tried one from Biogenex with the same results. Can anyone give any suggestions

Carolyn Luty
Montreal General Hospital

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