just to muddy the waters a bit more.
we find that fresh cut sections give much more stronger staining with ER-alpha
antibodies than sections that have been cut and stored a while.
abizar
www.innogenex.com
1-877-igx-info

Dana Settembre wrote:

> On Wed, 19 Jul 2000 carolyn.luty@muhc.mcgill.ca wrote:
>
> >
> >
> > We are having problems with very weak staining with ER. We use antigen retrieval
> > in citrate buffer in the microwave.The antibody was from Nova-Castra and then we
> > tried one from Biogenex with the same results. Can anyone give any suggestions
> > ??.
> >
> > Carolyn Luty
> > Montreal General Hospital
> >
> >
> >
>
> Hi Carolyn,
> I use Dako's ER Clone 1D5, code# M7047
> I preheat in Dako's Target Retieval System(TRS) in a steamer for 30min,
> then steam the slides in that TRS for 30min and cool down for 20 min
> I use the antibody at a 1:10 dilution and incubate in it for 60 minutes.
> I had lots of trouble getting it to work until I did it this way.
> Good Luck.
> Dana Settembre
> Immunohistochemistry Lab
> Pathology Department
> University Hospital
> Newark,  New Jersey
> USA