Re: ER

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From:Tom Wells <>,
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> We are having problems with very weak staining with ER. We use antigen
> in citrate buffer in the microwave.The antibody was from Nova-Castra and
then we
> tried one from Biogenex with the same results. Can anyone give any
> ??.
> Carolyn Luty
> Montreal General Hospital
I have two suggestions:

Firstly, check the pH of the citrate buffer. We found that the pH would
drift with time and that was often the cause of poor staining.

Secondly, look at your antigen retrieval protocol.  We use the Novacastra
antibody, however, our retrieval method consists of using Dako's Target
retrieval solution in an Autoclave.

I hope this helps. Tom

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