On Wed, 19 Jul 2000 carolyn.luty@muhc.mcgill.ca wrote:

> 
> 
> We are having problems with very weak staining with ER. We use antigen retrieval
> in citrate buffer in the microwave.The antibody was from Nova-Castra and then we
> tried one from Biogenex with the same results. Can anyone give any suggestions
> ??.
> 
> Carolyn Luty
> Montreal General Hospital
> 
> 
> 

Hi Carolyn,
I use Dako's ER Clone 1D5, code# M7047
I preheat in Dako's Target Retieval System(TRS) in a steamer for 30min, 
then steam the slides in that TRS for 30min and cool down for 20 min
I use the antibody at a 1:10 dilution and incubate in it for 60 minutes.
I had lots of trouble getting it to work until I did it this way.
Good Luck.
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey
USA