Re: ER
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From: | a i d a n s c h u r r <Aidan.Schurr@hvh.co.nz> |
To: | carolyn.luty@muhc.mcgill.ca, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Carolyn,
My guess would be that the antigen retrieval is to blame. We had
a problem like this recently - I discovered the citrate wasn't getting
hot enough to retrieve efficiently. Have a play with that and see if it
helps!!
Good luck
Aidan
>
> We are having problems with very weak staining with ER. We use antigen retrieval
> in citrate buffer in the microwave.The antibody was from Nova-Castra and then we
> tried one from Biogenex with the same results. Can anyone give any suggestions
> ??.
>
> Carolyn Luty
> Montreal General Hospital
>
>
>
___________________________________________________
shin: device for finding furniture in the dark...
___________________________________________________
a i d a n c s c h u r r
mlso, histology department
hutt valley health
lower hutt, new zealand
ph. ++64 4 5709173
fax ++64 4 5709214
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