Re: ER

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From:a i d a n s c h u r r <Aidan.Schurr@hvh.co.nz>
To:carolyn.luty@muhc.mcgill.ca, histonet@pathology.swmed.edu
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Carolyn,

My guess would be that the antigen retrieval is to blame.  We had 
a problem like this recently - I discovered the citrate wasn't getting 
hot enough to retrieve efficiently.  Have a play with that and see if it 
helps!!

Good luck
Aidan
> 
> We are having problems with very weak staining with ER. We use antigen retrieval
> in citrate buffer in the microwave.The antibody was from Nova-Castra and then we
> tried one from Biogenex with the same results. Can anyone give any suggestions
> ??.
> 
> Carolyn Luty
> Montreal General Hospital
> 
> 
> 


___________________________________________________
shin: device for finding furniture in the dark...
___________________________________________________
a i d a n   c   s c h u r r 
     mlso,  histology department
      hutt valley health
       lower hutt, new zealand

     ph.  ++64 4 5709173
     fax  ++64 4 5709214
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