Denise,
  I have an autostainer and I use a buffer from Signet Pathology Systems 
which is a 20x concentrate.  It seems to work o.k. and as far as I know has 
given no problems.  As far as the antibody diluent, yes I do use Dako's 
brand (S8309) and I am very satisfied with it.

Carla Shoffeitt
Columbus Regional Medical Center
Columbus, GA.


>From: denise M m Long-Woodward <denisew2@juno.com>
>To: histonet@pathology.swmed.edu
>Subject: Dako autostainer
>Date: Mon, 24 Jul 2000 10:03:15 +0000
>
>I've been asked to relay a question to all those who work with Dako's
>Autoimmunostainer. What are you using for Buffer rinses: Dako's
>commercial  TBS or something else?  Those who use something else, could
>you please tell me what it is?   They also want to know what you are
>using to dilute your antibodies. Are you using Dako's commerical stuff?
>Again please specify the diluent being used.
>
>Many thanks for your help!
>
>Denise Long Woodward
>________________________________________________________________
>YOU'RE PAYING TOO MUCH FOR THE INTERNET!
>Juno now offers FREE Internet Access!
>Try it today - there's no risk!  For your FREE software, visit:
>http://dl.www.juno.com/get/tagj.
>

________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com