Re: Carazzi hematoxylin
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From: | Ken Turner <ken.turner@stonebow.otago.ac.nz> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; format=flowed; charset=us-ascii |
Gayle (or anyone else),
Perhaps you can clear up some confusion regarding the correct proportions
of haematoxylin for Carazzi's formula. In the third edition of Bancroft he
cites 0.5g hx for a final solution of 500cc and doubling the mix to 1g for
a rapid method. However, in Bancroft 4th edition he cites using 5g hx for a
final solution of 500cc and doubling (10g) or even trebling (15g) for more
rapid staining. Which is the most suitable mix?
Ken.
At 22:12 6/07/00 +0000, you wrote:
>The recipe is found in Bancroft and Stevens, Theory and Practice of
>Histological Techniques, if you have a copy handy.
>
>
>Gayle Callis
>Veterinary Molecular Biology
>Montana State University
>Bozeman MT 59717-3610
>406 994-4705
>406 994-4303
Kenneth W Turner
Senior Teaching Fellow
Manager Histology Service Unit
Department of Pathology
Dunedin School of Medicine e-mail ken.turner@stonebow.otago.ac.nz
University of Otago Phone: (03) 479 7135 or (03) 479-7152
New Zealand Fax: (03) 479-7136
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