RE: peripheral nerve staining

<< Previous Message | Next Message >>
From:"Smith, Allen" <>
To:'Louise Taylor' <>
Content-Type:text/plain; charset=iso-8859-1

There are 2 very sensitive silver methods for peripheral nerves.  
Winkelman's method is reliable and gives exquisite results, but it takes
about 2 months! (Winkelman, R.K., 1960. Nerve Endings in Normal and
Pathologic Skin, p. 159. Charles C. Thomas, Springfield, Illinois.)
Kiernan's method can be done in an afternoon, but it is tricky and takes
practice to do well.  Expect to do it 3 times before you learn to do it
right. Kiernan, J.A., 1990. Histological and Histochemical Methods, 2nd ed.,
pp. 312-314 OR 3rd ed., pp. 371-373.  Butterworth Heinemann, Oxford,

-----Original Message-----
From: Louise Taylor []
Sent: Wednesday, July 19, 2000 9:04 AM
To: histonet
Subject: peripheral nerve staining

Hi Histonetters,

Seeing as there have been some queries regarding  neuropathology I thought I
might as well jump on  the bandwagon myself with a question.
A colleague is trying to demonstrate peripheral nerves in 2 day old rats for
subsequent image analysis. I have tried S100, but have got variable results
as well as some tissue damage due to microwave antigen retrieval (poor
quality IHC without HIER) so.... can anyone suggest something that might be
more useful? Perhaps a special stain? One of the old fashioned silver ones?

looking forward to your suggestions
Louise taylor

Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
South Africa

<< Previous Message | Next Message >>